Microfertilization, or ICSI, or intracellular sperm injection, is a well-chosen sperm procedure, in which all the sperm, according to the sperm chart, are either poorly motile or malformed.
In addition, the method is used when conventional IVF methods have failed.
ICSI Microfertilization – Procedure
During the process of application of micro-fertilization, a single sperm is selected, which is injected in a special way into the egg so that the second one can be fertilized in vitro.
It is the case in which a sperm is placed inside the cytoplasm of the egg.
The process of fertilizing the egg, placed on a special plate in the laboratory, takes a few hours.
When fertilization is achieved then it is placed in the woman’s uterus to start the life of the fetus.
The procedure involves creating a hole in the area that improves the chances of normal implantation of the fetus in the uterus.
Couples at high risk of having an abnormal chromosome number or a history of simple gene defects or chromosome defects are ideal candidates for this procedure.
It is used to diagnose a large number of genetic defects at present.
There are differences between the classic IVF and ICSI methods. However, the steps to follow before and after insemination are the same.
In terms of fertilization, ICSI only needs one sperm cell per oocyte.
Once fertilized, the egg transforms into a pre-embryo that must be transferred to the uterus to continue its development.
Defective sperm function remains the most important cause of human infertility.
Although some serious forms of male infertility have a genetic origin, others may be the result of environmental factors.
Over the past decade, ICSI has been increasingly used around the world to alleviate the problems of severe male infertility in people who either could not be helped by conventional IVF procedures or could not be accepted for IVF because there were too many few kinetically and morphologically normal sperm in the ejaculate of the male partner.
Low sperm count, poor sperm quality and inability to fertilize are some of the factors for which ICSI is recommended.
The procedure is often used in DNA fragmentation, when the mother’s age is advanced or when the sperm quality is poor and very good control is required to retrieve the most suitable sperm.
The method has helped thousands of couples around the world to have children of their own, despite the fact that the man may have serious infertility problems.
The method is also followed in cases of teraspermia.
This detects the few sperm that have a normal morphology allowing the best success rate to be achieved.
ICSI is generally performed following an online oocyte retrieval procedure, to extract one to several oocytes from a woman.
In ICSI IVF, the man or donor provides a sperm sample the same day the eggs are collected.
The sample is tested in the laboratory and if there is no sperm, doctors will remove the sperm from the epididymis or testicle.
Sperm extraction from epididymitis is also known as epidermal sperm inhalation (PESA) and testicular sperm extraction is also known as testicular sperm inhalation (TESA).
The procedure is performed under a microscope in a specially equipped laboratory.
A retaining forceps stabilizes the mature oocyte with gentle suction applied by a microinjector.
On the opposite side, a thin, hollow glass slot is used to collect a single sperm.
The oocyte is penetrated and the sperm is directed to the inside of the oocyte (cytoplasm).
The sperm is then released into the oocyte.
After the procedure, the oocyte will be placed in a cell culture and checked the next day for fertilization sites.
In natural fertilization, on the other hand, the sperm competes and when the first sperm penetrates the egg, it hardens to prevent the entry of any other sperm.